BIO 3070 Cell Culture Techniques
This lecture/laboratory course will provide students with a solid understanding of basic sterile cell culture techniques through the growth and maintenance of both normal and transformed adherent and suspension cell in culture. Topics include primary and explant culture techniques, trypsinization and media supplementation, cell counting, determining viability and growth curves in plate and well cultures, single cell cloning, transfection technologies, fluorescent analysis, photomicrography, and reporter assays, cryopreservation, cell cycle determinants and the induction of apoptosis. This course is strongly recommended for those students who plan to do cell or tissue work in cancer or neuroscience research for their senior project.